Fig. 3: PCP and METH induce gain of GAD1 in the same PL glutamatergic neurons.

a Mouse line used for tamoxifen-inducible genetic labeling. b Examples of neurons that are GAD1⁺/VGLUT1⁺/TdTom⁺, GAD1⁺/VGLUT1⁺, and VGLUT1⁺/TdTom⁺. Scale bar, 10 μm. c Experimental protocol to distinguish VGLUT1 neurons co-expressing GAD1 in drug-naïve conditions from those gaining GAD1 upon PCP treatment. d Quantification of the neurons shown in b in mice treated as described in c (n = 4 mice). e Experimental protocol to learn whether serial administration of PCP and METH changes the transmitter phenotype of the same number of neurons as PCP alone, causes neurons that have gained GAD1 to lose it, or enables other neurons to gain GAD1. f Quantification of neurons shown in b in mice treated as described in e (n = 4 PCP + saline, 4 PCP + PCP, 5 PCP + METH-treated mice). Statistical significance (****P < 0.0001) was assessed using and two-way ANOVA followed by Tukey’s test. Data are presented as mean ± SEM. The exact p-values and additional statistical details can be found in Supplementary Table 3.