Fig. 7: Suppression of drug-induced PL hyperactivity after the end of drug-treatment reverses the change in transmitter identity and reverses drug-induced behavioral alterations.

a Experimental protocol. Cartoon after⁷⁵. b PL expression of VGLUT1 and GAD1 after exposure to saline/drug treatment followed by chemogenetic activation of PV⁺ neurons. (Blue rectangles) higher magnification of GAD1⁺/VGLUT1⁺ neurons in insets. Scale bar, 50 μm. c Quantification of (b) (n= from left to right 6, 4, 4, 5, 4, 4 mice). d–i PCP- and METH-induced locomotor sensitization and deficits in the NORT and SAT are reversed by sustained activation of PV⁺ neurons (d n= from left to right 6, 4, 7, 6 mice; e n= from left to right 6, 4, 8, 7 mice; f n= from left to right 7, 4, 8, 6 mice; g n= from left to right 8, 7, 9, 8 mice; h n= from left to right 9, 8, 8, 9 mice; i n= from left to right 9, 7, 9, 9 mice). Statistical significance (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001) was assessed using two-way ANOVA followed by Tukey’s test. Data are presented as mean ± SEM. The exact p-values and additional statistical details can be found in Supplementary Table 7.