Fig. 2: Effect of KlebCP on the molar mass distribution in PVC samples.
From: Revisiting the activity of two poly(vinyl chloride)- and polyethylene-degrading enzymes
The figure shows the results of SEC analysis of high molar mass (panels A, C) and low molar mass (panels B, D) PVC samples treated with 10 µM KlebCP in the absence or presence of an H2O2 source. 50 nM cellobiose dehydrogenase (CDH) was used alongside 5 mM cellobiose to generate hydrogen peroxide in situ. The reactions were incubated at 30 °C for 96 h in 50 mM Tris-HCl buffer, pH 8.0, supplemented with 300 mM NaCl and 100 mg/mL PVC powder. Circular markers in panels A and B indicate the results obtained for independent reactions. Black horizontal lines denote average values whereas error bars represent standard deviations between triplicates. Mw, weight average molar mass; Mn, number average molar mass. Panels C and D show chromatograms resulting from SEC-MALS (multi-angle light scattering) analysis of the samples. Solid lines indicate the differential refractive index detector response, whereas dotted lines represent the molar mass data. Note that the chromatographic traces are overlapping almost completely. Source data are provided as a Source Data file.
