Fig. 4: Probing the phenoloxidase activity of “Ceres” on L-DOPA.
From: Revisiting the activity of two poly(vinyl chloride)- and polyethylene-degrading enzymes
The figure shows the formation of dopachrome (panel A) and the depletion of molecular oxygen (panel B) as the result of L-DOPA oxidation by “Ceres” or by a tyrosinase from Agaricus bisporus (AbTyr). The reactions were carried out at room temperature in 20 mM HEPES, pH 7.0, supplied with 150 mM NaCl and 5% (v/v) glycerol, using various concentrations of enzymes and L-DOPA. Error bars in panel A represent standard deviations for replicates (n = 3) and in most cases are hidden behind the data markers indicating average values. Note that the data markers for the negative control reaction (L-DOPA + buffer) and the data markers for the experiment with “Ceres” are overlapping on panel A. The oxygen depletion experiments (panel B) were initiated by injecting enzymes or reaction buffer (negative control) through self-sealing septa into air-tight reaction vessels containing the substrate. The injections occurred at ~15 min of incubation, as indicated by the black arrow. The reactions shown in panel B were performed twice and all data points are shown. Source data are provided as a Source Data file.
