Fig. 7: Phenoloxidase activity in wax worm saliva (GmSal) before and after heat inactivation.
From: Revisiting the activity of two poly(vinyl chloride)- and polyethylene-degrading enzymes
The graph shows endpoint data from 12 h reactions and demonstrates the conversion of L-DOPA to dopachrome, which is reflected in an increase in absorbance at 490 nm. As saliva concentration is increased, dopachrome production increases, indicating phenoloxidase activity. Note that the reactions with heat-treated saliva (“inactivated”) show essentially no conversion of L-DOPA. The experiments were carried out at 24 °C using 1 µM of tyrosinase from Agaricus bisporus (positive control) or 1–5 µL of GmSal in 20 mM HEPES, pH 7.0, supplied with 4 mM L-DOPA, 150 mM NaCl and 5% v/v glycerol. Circular markers indicate individual results obtained for independent reactions (n = 3). Black horizontal lines denote average values whereas error bars represent standard deviations. Source data are provided as a Source Data file.
