Fig. 8: Radioreduction levels in vitro and in vivo before and after treatment of Ptc-PA.
From: Atomic-scale strain engineering of atomically resolved Pt clusters transcending natural enzymes
a Survival rate of CHO cells after coculture of Ptc-PA with different concentrations (3, 6, 12, 25, 50, 100, 200, 300 μg/mL) for 24 h and 48 h (n = 6 per group). b In vitro protection effect of Ptc-PA with different concentrations (n = 3 per group). Data are presented as mean ± SEM, analyzed by one-way ANOVA with one-sided Tukey’s multiple comparisons test (the p values are shown). Fluorescence microscopic images of intracellular (c) ROS and (d) O2·− levels under different conditions. Experiments were repeated independently five times with similar results. e Fluorescence quantification of CHO cells staining for ROS and O2•− (n = 5 per group). Data are presented as mean ± SEM, analyzed by one-way ANOVA with one-sided Tukey’s multiple comparisons test (the p values are shown). Quantitative analysis of intracellular (f) ROS and (g) O2·− levels under different conditions by flow cytometry. h Cell apoptosis of CHO cells stimulated by 800 μM H2O2 and 1 mg/mL LPS with and without Ptc-PA treatment. i Statistical diagram of early apoptosis and late apoptosis obtained from cell apoptosis test (n = 3 per group). Data are presented as mean ±SEM, analyzed by one-way ANOVA with one-sided Tukey’s multiple comparisons test (the p values are shown). j Schematic diagram of Ptc-PA modulated radiation-induced oxidative damage (Created with BioRender.com). k Survival percentage of irradiated mice with or without treatment of Ptc-PA, as well as Amifostine as a control. l The level of WBC and PLT of mice with or without the treatment of Ptc-PA (n = 3 per group). Data are presented as mean ±SEM, analyzed by one-way ANOVA with one-sided Tukey’s multiple comparisons test (the p values are shown). m The level of DNA and BMNC of mice with or without the treatment of Ptc-PA (n = 5 per group). Data are presented as mean ± SEM, analyzed by one-way ANOVA with one-sided Tukey’s multiple comparisons test (the p values are shown). The level of (n) Lung SOD, Liver SOD, (o) Lung MDA and Liver MDA of mice with or without the treatment of Ptc-PA (n = 3 per group). Data are presented as mean ±SEM, analyzed by one-way ANOVA with one-sided Tukey’s multiple comparisons test (the p values are shown).
