Fig. 6: Some developing neuronal cells in the gastrodermis are labelled by vasa2::mOr2 or piwi1P2A-GFP.
From: A population of Vasa2 and Piwi1 expressing cells generates germ cells and neurons in a sea anemone
Confocal live imaging of the parietal muscle region from vasa2::mOr2/prdm14d::GFP (A-A’’) or piwi1P2A-GFP/soxB(2)::mOr2 (B-B’’) double transgenic reporter lines at the mid-body level of juvenile polyps (see C). A-A’’ Differentiated gastrodermal neurons in the parietal muscle express [prdm14d::GFP]high but no vasa2::mOr2 protein (black arrowheads). In contrast, [prdm14d::GFP]low colocalizes with [vasa2::mOr2]low in cells along the parietal muscle and in the body wall gastrodermis (arrows). B-B’’ Similarly, neuronal cell bodies within axonal bundles along the parietal muscle show high levels of the neural progenitor marker gene soxB(2)::mOr2 but no GFP protein (black arrowheads). While some [GFP]high cells show no soxB(2)::mOr2 (asterisks), relatively low levels of soxB(2)::mOr2 are detected in a subset of [GFP]low cells with neuron-like shape (arrows). C 3-dimensional illustration of a juvenile body column section highlighting the gastrodermal ___location of neural (yellow) among other somatic progeny (orange) that potentially derive from mesenterial Vasa2+/Piwi1+ stem/progenitor cells (blue). Arrows indicate putative migration of transit-amplifying progenitors. pm parietal muscle. Scale bars: 10µm. Experiments performed twice with similar results.
