Fig. 9: In vivo long-term immune memory induced by RMn-NB-sensitized RT.
a Schematic illustration of in situ vaccination mediated by RMn-NB-sensitized RT. Created in BioRender. Luo, Z. (2024) BioRender.com/n85g329. b Tumor growth curves, (c) isolated tumor photographs, and (d) tumor weights of BALB/c mice immunized with Saline + RT and RMn-NBs + RT-treated CT26 tumor cells, respectively (n = 6 mice). e Percentages of TEM in the spleen analyzed by FCM. The spleens in different groups are collected on day 30 (n = 6 mice). f Tumor growth curves of 4T1 breast tumor-bearing mice treated with Saline, Saline + RT, RMn-NBs + RT, RMn-NBs + RT + αCD8a and RMn-NBs + RT + αPD-L1 (n = 6 mice). The doses of Mn2+ and RANa are 5.5 mg kg−1 and 30.5 mg kg−1, respectively. X-ray irradiation is performed after intravenous injection of RMn-NBs (10 mM × 0.2 mL) for 6 h. Treatments are performed with 3 fractions (total 6 Gy) on days 0, 3, and 6. αCD8a (10 mg kg−1) and αPD-L1 (10 mg kg−1) are respectively administered via intraperitoneal injection after RT for 6 h, and treatments are performed on days 0, 3, and 6 (n = 6 mice). g Weights of 4T1 breast tumor after various treatments (n = 6 mice). h Survival curves of 4T1 breast tumor-bearing mice (n = 6 mice). i Quantification of metastatic lesions in the lungs (n = 6 mice). j Average size of each nodule in the lungs (n = 6 mice). All data are shown as mean ± SD. Statistical analysis is performed using two-tailed Student’s t-test for comparing two groups, and one-way ANOVA analysis of variance for multiple groups. p > 0.05 represent nonsignificance, while p < 0.05 indicate statistical significance. Source data are provided as a Source Data file.
