Fig. 6: B. acidifaciens synthesizes pentadecanoic acid (C15:0). | Nature Communications

Fig. 6: B. acidifaciens synthesizes pentadecanoic acid (C15:0).

From: Galactooligosaccharides and Limosilactobacillus reuteri synergistically alleviate gut inflammation and barrier dysfunction by enriching Bacteroides acidifaciens for pentadecanoic acid biosynthesis

Fig. 6

a Pentadecanoic acid concentrations in the supernatant of B. acidifaciens culture supplemented with or without GOS, L. reuteri, or their combination (n = 4 per group). b Metabolic pathway of C15:0 in B. acidifaciens. c Relative abundances (%) of bacterial genes involved in C15:0 synthesis based on shotgun metagenomic analysis (n = 6 per group). d Spearman two-tailed correlation analysis between Bacteroides spp. and representative fecal metabolites. e, f Linear regression analysis between fecal concentrations of C15:0 and relative abundance of B. acidifaciens in the feces of mice following transplantation with fecal microbiota (FMT, n = 8 per group) or fecal bacterial cell-free supernatants (FST, n = 6 per group). One-way ANOVA with Tukey’s test was used for (a), and Kruskal-Wallis test and post-hoc Dunn’s test was used for (e, f). Data were presented as mean ± SEM. Source data were provided as a Source Data file. ACP acyl carrier protein, accC acetyl-CoA carboxylase, acrC enoyl-ACP reductase C, acs acetyl-CoA synthetase, FAS2 fatty acid synthase 2, FASN fatty acid synthase system, FATA fatty acyl-ACP thioesterase A, fabA 3-hydroxyacyl-ACP dehydratase, fabB 3-oxoacyl-ACP synthase I, fabD ACP-S-malonyltransferase, fabF 3-oxoacyl-ACP synthase II, fabG 3-oxoacyl-ACP reductase, fabH 3-oxoacyl-ACP synthase III, fabK enoyl-ACP reductase II, fabL enoyl-ACP reductase I, fabM trans-2-decenoyl-ACP isomerase, fabZ 3-hydroxyacyl-ACP dehydratase, fadD long-chain acyl-CoA synthetase, MCH medium chain hydrolase, paaL acyl-CoA thioesterase, pccB propionyl-CoA carboxylase beta chain.

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