Fig. 5: The oncoprotein-binding sites change during B-ALL-to-AML lineage-switching events.
From: MLL oncoprotein levels influence leukemia lineage identities
a Boxplot comparing the oncoprotein scores in a patient matched B-ALL and AML sample that lineage switched in response to B-ALL directed therapy. The p value was computed using a two-tailed independent samples t-test; n = 1692 oncoprotein-target sites; boxplot center lines = median, box limits = first and third quartiles, whiskers = 1.5 times the interquartile range (IQR). b Oncogene expression is significantly reduced in the AML after lineage switching. Bar height is the average of three qPCR biological replicates; Error bars = standard deviation, and the p value was computed using a two-tailed independent samples t-test; n = 3 qPCR biological replicates. c Genome Browser tracks showing the oncoprotein levels are elevated over the BANK1 locus in the B-ALL sample prior to lineage switching. Bottom black bars indicate B-ALL specific oncoprotein target regions. d B-ALL specific oncoprotein target genes are expressed at significantly higher levels in the B-ALL sample (gold) prior to lineage switching than the AML sample (pink). Bar height is the average of three qPCR biological replicates; Error bars = standard deviation; p values computed using a two-tailed independent samples t-test; n = 3 qPCR biological replicates. e Genome Browser tracks showing the oncoprotein levels are elevated over the FNDC3B locus in the AML sample after lineage switching. Bottom black bars indicate AML specific oncoprotein target regions. f Same as (d) but for AML-specific oncoprotein target genes. g A Venn Diagram comparing the lineage-switching B-ALL-specific and AML-specific oncoprotein-target genes to all other oncoprotein-target genes. The AML group includes 51 non-overlapping genes referred to as the GMP-like program. h The UMAP embedding of healthy lineage-depleted bone marrow from Otto et al. ref. 23, colored by the average z-scores of the 51 non-overlapping AML-specific oncoprotein target genes from (g). Source data are provided as a Source Data file.
