Fig. 4: Host transcriptional responses to identify determinants of Mtb restriction in GID/CTLH knockout macrophages. | Nature Communications

Fig. 4: Host transcriptional responses to identify determinants of Mtb restriction in GID/CTLH knockout macrophages.

From: Genome-wide screen of Mycobacterium tuberculosis-infected macrophages revealed GID/CTLH complex-mediated modulation of bacterial growth

Fig. 4

A Principal component analysis (PCA) of scramble or GID8−/−, MAEA−/− and WDR26−/− knockout host (BMDMhs) transcriptomes infected with the Mtb smyc’::mCherry strain at MOI 0.5 4 days post infection. B, C Venn diagrams of upregulated and downregulated gene sets (Supplementary Data 4) in GID8−/−, MAEA−/− and WDR26−/− BMDMhs as compared to scramble showing overlapping genes. Differentially expressed genes cutoff; abs (log2 fold change) >0.5, adjusted p value < 0.05. D Tree plot of top 50 enriched gene ontology terms (biological process) amongst the 1267 commonly upregulated genes in GID8−/−, MAEA−/−, and WDR26−/− BMDMhs.

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