Fig. 7: Mtb infected GID/CTLH knockout macrophages are more autophagic and anti-inflammatory. | Nature Communications

Fig. 7: Mtb infected GID/CTLH knockout macrophages are more autophagic and anti-inflammatory.

From: Genome-wide screen of Mycobacterium tuberculosis-infected macrophages revealed GID/CTLH complex-mediated modulation of bacterial growth

Fig. 7

A Western blot analysis of autophagic LC3I to LC3II turnover in uninfected and Mtb infected scramble or GID8−/−, MAEA−/−, and WDR26−/− BMDMhs at indicated MOIs. B Densitometry quantification (in %) of LC3I to LC3II turnover (LC3II/LC3I ratio) in (A) and Fig. S11A, B as normalized to β-actin; n = 3 biological replicates. *P < 0.05; **P < 0.01; ****P < 0.0001, two-way ANOVA alongside Dunnett’s multiple comparison test. Data are presented as mean values ± SD. C Analysis of the expression of interferon 1 beta (IFN-β) in scramble or GID8−/−, MAEA−/− and WDR26−/− Mtb infected macrophages. BMDMhs were infected at MOI 4, and RNA was extracted for qPCR at 4 and 24 hours post infection; n = 3 biological replicates. *P < 0.05; ****P < 0.0001, two-way ANOVA alongside Dunnett’s multiple comparison test. Data are presented as mean values ± SD. ELISA was carried out in culture supernatants 24 hours post infection; n = 4 biological replicates. ****P < 0.0001, one-way ANOVA alongside Dunnett’s multiple comparison test. Data are presented as mean values ± SD. Source data are provided as a Source Data file.

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