Fig. 6: Reactivity-based antigen profiling for the discovery of reactive cysteines on MHC-I-bound antigens.
From: A platform for mapping reactive cysteines within the immunopeptidome
a Schematic representation of the reactivity-based antigen profiling workflow. b Violin plot comparing the predicted MHC-I binding ranks of MS-DTB-enriched 8–10-mer peptides in MT2 parental and HLA knockout cells. The statistical significance was evaluated through unpaired two-tailed Student’s t-tests. P values were 1.2 × 10−5 and 6.2 × 10−8. The result is a representative of two experiments (n = 2 independent replicates). c Motif analysis of MS-DTB-modified 9-mer peptides identified in MT2 parental cells. The result is a representative of two experiments (n = 2 independent replicates). d Validation of MS-DTB-modified peptides using SCTs. The result is a representative of two experiments (n = 2 independent replicates). e. Modeling studies indicate that cysteines in the MS-DTB-modified peptides are solvent-exposed when forming pMHC-I complexes. Source data are provided as a Source Data file.
