Fig. 7: Targeting reactive cysteines on MHC-I-bound antigens to induce ADCP.
From: A platform for mapping reactive cysteines within the immunopeptidome
a Structure of M-Fc-III-4C. b Schematic representation of anti-CD20-induced antibody-dependent cellular phagocytosis (ADCP) between Jurkat ADCP reporter cells and Raji cells. IgG, immunoglobulin G. c ADCP assay measuring luciferase activation in Jurkat-Luc NFAT-CD32 cells after co-culturing with Raji cells in the presence of anti-CD20 antibody. Anti-β-galactosidase (Gal) antibody served as a negative control. Data represent mean values ± SEM (n  =  3 independent replicates). The statistical significance was evaluated through unpaired two-tailed Student’s t-tests. P values were 0.000078 and 0.000075. d Schematic representation of targeting reactive cysteines on MHC-I-bound antigens to induce ADCP. e ADCP assay measuring luciferase activation in Jurkat-Luc NFAT-CD32 cells after co-culturing with M-Fc-III-4C-treated MT2 parental and HLA knockout cells in the presence of human IgG. Data represent mean values ± SEM (n  =  3 independent replicates). The statistical significance was evaluated through unpaired two-tailed Student’s t-tests. P values were 0.0066 and 0.00027. Source data are provided as a Source Data file.
