Fig. 3: Development and characterization of CTA 1.0 in S. A-14.
From: Repurposing endogenous type I-E CRISPR-Cas systems for natural product discovery in Streptomyces
a Schematics of CRISPR-based transcriptional activator 1.0 (CTA 1.0) in this work. The activation ___domain was fused to the Cascade (casA-casB-cas7-cas5-cas6) via a linker at the CasA N-terminus. b CrRNAs targeting the PTM-ctg-610 promoter region of the PTM BGC are shown. c HPLC analysis for activating PTM BGC using the CTA 1.0 and CTA 2.0 in S. A-14. (★ indicates major products of the PTM BGC). d The structures of the compound PTM-a ~ c. Control (The wild-type strain of S. A-14). Source data are provided as a Source Data file.
