Fig. 5: Activation of C11 biosynthetic gene cluster in Streptomyces A-14.
From: Repurposing endogenous type I-E CRISPR-Cas systems for natural product discovery in Streptomyces
a CrRNAs targeting the promoter regions of ctg-949 gene are shown. b HPLC analysis for activating C11 BGC using the CTA 2.0 in S. A-14. c The structure of the compound secalonic acid. A2-4 (Activation strains with kasO*p driven the expression of the Sav Cascade and MS2-coupled SoxS ___domain); ΔC11(Strains with cluster 11 deleted in the chromosome of S. A-14, as a control); ΔC11-c1(The cluster-deletion strain with activation tool applied to target the promoter regions of ctg-949 gene, as a control); Control (The wild type strain of S. A-14). Source data are provided as a Source Data file.
