Fig. 2: Rice importin α-mediated transport of MoHTR1. | Nature Communications

Fig. 2: Rice importin α-mediated transport of MoHTR1.

From: Nuclear localization sequence of MoHTR1, a Magnaporthe oryzae effector, for transcriptional reprogramming of immunity genes in rice

Fig. 2

A Yeast two-hybrid assay of the MoHTR1 and importin α proteins of the rice blast fungus (Moimpα) and rice (Osimpα, Osimpα1a, and Osimpα1b). Interaction transformants were cultured with different dilutions on selection media. B In vivo interaction between MoHTR1 and rice importin α proteins. MoHTR1, NLS deleted MoHTR1 (MoHTR1∆NLS), and rice importin αs with split YFP were expressed under Ubiquitin promoter. BiFC assay was performed by co-transfecting of nYFP fused plasmid and cYFP fused plasmid in the rice protoplast. Scale bar; 10 μm. C Nuclear localization proportion of MoHTR1 in rice wild type cultivar and OsImpα1a T-DNA insertion mutant. Mean ± SD, n = 3 independently transfected protoplasts, significance was determined by an unpaired two-tailed Student’s t-test (**p < 0.01 and ***p < 0.001). Representative data are shown from independently experiments and source data are provided as a Source Data file.

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