Fig. 6: Bcl11b degradation by the OsTIR1-AID2 system.

a Expression of Bcl11b in total thymocytes analyzed by flow cytometry 16 h after a single-dose intraperitoneal injection of PBS or 5-Ph-IAA. Data are representative of three independent experiments. b Thymocytes were analyzed by flow cytometry after 1, 2, and 3 days of daily 5-Ph-IAA treatment or 3-day treatment of PBS. Data are representative of three independent experiments. A schematic diagram for gating the indicated thymocyte populations is shown at the bottom. c Flow cytometric analysis of thymocytes in mice treated with 5-Ph-IAA for 3 days. Data are representative of three independent experiments. d–f Pregnant Rosa26^{LSL-OsTIR1/ LSL-OsTIR1};Bcl11b^AID/AID female mice mated with Bcl11b^AID/AID;VavCre male mice received intraperitoneal injection of 5-Ph-IAA at 13.5 and 14.5 days of gestation (d). The total lung ILC2 (CD45⁺CD3^–Thy1.2⁺ST2⁺) numbers were examined in the offspring at 3 weeks (e) and 9 weeks (f), by flow cytometry (n = 3 for each group; p = 0.02488 and 0.0005357 for ST2⁺ and ST2⁺KLRG1⁺ ILC2 at 3 weeks). Data are mean ± s.e.m. Statistical analysis was performed using two-sided unpaired t-test. *p < 0.05, **p < 0.005. Source data are provided as a Source Data file.