Fig. 6: SNORD116-specific genes may be crucial for PWS phenotypes.
From: Roles of SNORD115 and SNORD116 ncRNA clusters during neuronal differentiation
A A heatmap representing the expression of SNORD116-specific genes in wildtype and mutant cells across differentiation. B Links between SNORD116-specific genes and PWS phenotype based on the published literature, supporting references are in the Supplementary Data 8. C UCSC genome browser view of transcription across clustered protocadherin genes gamma in wild-type and mutant cell lines at D15. Yellow boxes mark genes with the differential expression in H116 vs both wildtype and H115 and quantified in D. D Mean expression of unique exons of protocadherin gamma genes in RNA-seq data relative to common exon 2 at D15, marked yellow on C. Error bars represent standard deviation, p values obtained from Student t test. E, F Mean expression of EDIL3 (E) and P4HTM (F) mRNA, protein, and antisense non-coding transcript across differentiation. For transcriptomic data, statistical significance originates from EdgeR analysis (quasi-likelihood F-test followed by Benjamini-Hochberg correction for multitesting); for proteomic data, from DEP (empirical Bayes statistics followed by Benjamini-Hochberg correction for multitesting). Error bars represent standard deviation (SD). Number of biological replicates for each mutant and timepoint is provided in Supplementary Data 1. Source data, together with exact p values, are provided as a Source Data file. Below is the UCSC genome browser view of overlapping transcription from mRNA and ncRNA.
