Fig. 4: Oncometabolite 25-HC promoted breast cancer liver and lung metastasis. | Nature Communications

Fig. 4: Oncometabolite 25-HC promoted breast cancer liver and lung metastasis.

From: Tumor-derived miR-9-5p-loaded EVs regulate cholesterol homeostasis to promote breast cancer liver metastasis in mice

Fig. 4

a Tumor volume followed in indicated xenografted mice (two-way ANOVA, n = 5 mice per group). b, c Representative bioluminescence images and quantification of mice inoculated with indicated group (one-way ANOVA, n = 5 mice per group). d, e Representative IHC images showing Ki67 staining and the overall percentage of Ki67+ tumor cells (one-way ANOVA, n = 5 mice per group). Scale bar, 50 μm. f Schematic diagram of 25-HC treated mouse model. 231/Ctrl or 231/miR-9 KO cells injected at day 0. On day 10, mice were treated with vehicle or 25-HC (10 mg/kg) every other day. At day 35, tissue was harvested, imaged and fluorescence quantified. Experimental timeline outlined above quantified data. g, h Representative bioluminescence images and quantification of mice inoculated with indicated groups (one-way ANOVA, n = 5 mice per group). i Quantification of liver nodules (one-way ANOVA, n = 5 mice per group). j 25-HC levels of livers in each group by ELISA kit. (one-way ANOVA, n = 6 mice per group). Data are presented as mean ± s.d. Source data and exact P value are provided as a Source data file.

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