Fig. 5: Ch25h blockage inhibited BC liver and lung metastasis.

a LC-MS/MS analysis of 25-HC in serum in indicated CH25H liver conditional knock out (Ch25h^L–/–) and control (Ch25h^fl/fl) mice (unpaired two-tailed t test, n = 6 mice per group). b Total cholesterol (TC) and triglyceride (TG) levels were examined from CH25H liver conditional knock out (Ch25h^L–/–) and control (Ch25h^fl/fl) mice. (unpaired two-tailed t test, n = 5 mice per group). c Liver sections were stained with oil red O in Ch25h^–/– and Ch25h^fl/fl mice with E0771 xenograft. Scale bars, 100 μm. d Representative PET/CT imaging with ¹⁸F-FDG for accessing liver and lung uptake and quantification in indicated in CH25H liver specific KO and WT mice. e Representative bioluminescence images of metastasis site in liver and lung and quantification of mice inoculated with indicated groups (unpaired two-tailed t test, n = 5 mice per group). f Representative IHC images showing Ki67 staining and the overall percentage of Ki67⁺ tumor cells in indicated groups (unpaired two-tailed t test, n = 5 per group). Scale bar, 50 μm. g Immunoblot analysis of CH25H in liver in indicated groups (top), IHC and of CH25H in liver sections in indicated groups (bottom). Scale bar, 50 μm. h Representative bioluminescence images of metastasis site in liver and lung and quantification of mice inoculated with indicated groups (unpaired two-tailed t test, n = 5 per group). i Representative IHC images showing Ki67 staining and the overall percentage of Ki67⁺ tumor cells of liver and lung from indicated groups (unpaired two-tailed t test, n = 5 per group). Scale bar, 50 μm. j Representative PET/CT imaging with ¹⁸F-FDG for accessing liver and lung uptake and quantification in indicated mice. Data are presented as mean ± s.d. Source data and exact P value are provided as a Source data file.