Fig. 3: Histopathological analysis of clinical isolate pulmonary disease manifestations. | Nature Communications

Fig. 3: Histopathological analysis of clinical isolate pulmonary disease manifestations.

From: Single nucleotide polymorphisms are associated with strain-specific virulence differences among clinical isolates of Cryptococcus neoformans

Fig. 3

Three mice were infected with each of the clinical isolates and sacrificed at 17 days post infection. Lungs were inflated, excised, and fixed in 10% neutral-buffered formalin, and stored in 70% ethanol. Lungs were sectioned and stained with hematoxylin and eosin (H&E) or immunohistochemically with antibodies targeting iNOS, CD68, eosinophil protein X (EPX), or CD4/CD8 dual stain. Slides were scanned and a representative image was taken at 20x magnification (right) or of an entire lung lobe (left).

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