Fig. 1: PanK4 is an exercise target abundant in skeletal muscle and associated with metabolic dysregulation.

A PanK4 Ser63 phosphorylation (p-PanK4^Ser63) and its regulation by exercise or muscle contraction in skeletal muscle from humans and rodents indicated by phosphoproteomics^13,14. p-PanK4^Ser63 in human vastus lateralis before (Pre) and post-exercise indicated time-points into recovery from vigorous cycling exercise (B, n = 6), prolonged endurance cycling, sprint cycling and strength exercises (C, n = 8). D Pank4 mRNA in indicated male mouse tissues (n = 6). E mRNA abundance of indicated Pank isoforms determined by whole genome transcriptome analysis in male mouse skeletal muscle (n = 8). F abundance of PanK1, PanK2 and PanK4 protein in indicated tissues from male mice and representative Western blots (n = 4). D–F data are presented as mean values +/− SEM. Statistic: A significantly regulated phosphopeptides (adjusted P-values less than 0.05) were identified in larger data sets using LIMMA’s moderated t-statistics with the eBayes method (see Refs. ^13,14 for more detail). B repeated measures one-way ANOVA and C repeated measures two-way ANOVA with log2-transformed data and Šidák post hoc testing. Source data are provided as a Source Data file. AU = arbitrary units. Schematic in Fig. 1A was created in BioRender. Kleinert, M. (2024) BioRender.com/t99l144.