Fig. 2: Skeletal Muscle PanK4 regulates fatty acid oxidation.

A PanK4 protein abundance in indicated tissues from male PanK4 wildtype (WT) and muscle-specific PanK4 knockout (mKO) mice at age 28 weeks. B body composition at age 26 weeks (n = 8 for WT and n = 6 for mKO) of male PanK4 WT and PanK4 mKO mice. C, D, volcano plot of 508 detected metabolites and heatmap of indicated metabolites in TA muscle from male glucose-stimulated PanK4 WT and PanK4 mKO mice at age 28 weeks (n = 8 for WT and n = 6 for mKO). E overview of the Coenzyme A biosynthesis pathway. F palmitate oxidation in ex vivo basal or contracted soleus muscles from ad libitum fed male PanK4 WT and PanK4 mKO mice at age 12-19 weeks (n = 10). Citrate synthase activity and muscle triglyceride (TG) concentrations in soleus (G) and gastrocnemius (H) muscles from male glucose-stimulated PanK4 WT and PanK4 mKO mice at age 28 weeks (n = 8 for WT and n = 6 for mKO). Statistic: B two-tailed unpaired t-test within fat or lean mass; C, D two-tailed welch test; F two-way ANOVA; G, H, two-tailed unpaired t-test. Statistical analyses were conducted with log2-transformed data. B, F–H, data are presented as mean values +/− SEM. PanK1-3 pantothenate kinase 1-3, PPCS phosphopantothenate-cysteine ligase, PPCDC phosphopantothenoylcysteine decarboxylase, PPAT phosphopantetheine adenylyltransferase, DPCK = 3’-Dephospho-CoA kinase. Source data are provided as a Source Data file.