Fig. 3: PanK4 regulates acetyl-CoA and metabolic flexibility in skeletal muscle.

A acetyl-CoA determined by non-targeted metabolomics in indicated skeletal muscle from male PanK4 WT and PanK4 mKO mice weeks (n = 8 for WT and n = 6 for mKO). B targeted analysis of acetyl-CoA in gastrocnemius muscle from fasted and fed male (circles) and female (squares) PanK4 WT and PanK4 mKO mice (n = 5 for fast/WT, n = 4 for fed/WT, n = 4 for fast/mKO n = 4 for fed/mKO). C representative Western blots and quantification of pan-lysine acetylation in gastrocnemius muscle from male (circles) and female (squares) PanK4 WT and PanK4 mKO mice (n = 5). D–F targeted analysis of citrate and malonyl-CoA in gastrocnemius muscle from fasted and fed male (circles) and female (squares) (E, n = 5 for fast/WT, n = 4 for fed/WT, n = 4 for fast/mKO n = 4 for fed/mKO; F, n = 5) and in indicated muscle from fed male (G, n = 12) PanK4 WT and PanK4 mKO mice. G representative Western blots and quantification of pan-lysine malonylation in gastrocnemius muscle from male (circles) and female (squares) PanK4 WT and PanK4 mKO mice (n = 5). H activity of pyruvate dehydrogenase in the active form (PDHa) activity in gastrocnemius muscle from male (circles) and female (squares) PanK4 WT and PanK4 mKO mice (n = 7 for fast/WT, n = 7 for fed/WT, n = 6 for fast/mKO n = 7 for fed/mKO). A–H data are presented as mean values +/- SEM. Statistic: A–H, two-way ANOVA. Statistical analyses were conducted with log2-transformed data. Šidák post hoc testing was performed. Source data are provided as a Source Data file. AU = arbitrary units.