Fig. 3: Aging facilitates the recruitment of tissue-resident γδ17 and neutrophils.

a Proportions of lung γδT cells were measured by flow cytometry, gated on CD45+ cells. Each group contains six mice. Data expressed as % of γδT cells (mean ± SD). YC-AC (P = 0.01), AC-AM (P = 0.04), YM-AM (P = 3.5E−03). Significance was determined using one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001, ns no significance, P > 0.05. b Quantity of γδT cells in per 0.1 g lung. Each group contains six mice. Data expressed as number of γδT cells (mean ± SD). YC-AC (P = 0.02), AC-AM (P = 6.0E−04), YM-AM (P = 2.0E−04). Significance was determined using one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001, ns no significance, P > 0.05. c Volcano plot showing DEGs in AC/YC, AM/YM, YM/YC, and AM/AC comparison. Significance was determined using the “FindMarkers” functions of the Seurat package with the Wilcoxon Rank Sum test and adjusted by Bonferroni correction. Proportions of lung CD69 + γδT (d) and S1PR1 + γδT (e) cells from AC and YC mice were measured by flow cytometry, gated on CD45+CD3+γδTCR+ cells. Each group contains six mice. Data expressed as % of CD69 + γδT or S1PR1 + γδT cells (mean ± SD). P = 3.4E−04 (d); P = 0.03 (e). Significance was determined using an unpaired two-tailed Student’s t test. *P < 0.05. f Violin plot showing average expression levels of IL-17A in γδT cells in each group, based on the scRNA-seq data. g Proportions of lung IL-17A + γδT cells were measured by flow cytometry, gated on CD45+CD3+γδTCR+ cells. Each group contains six mice. Data expressed as % of γδ17 cells (mean ± SD). YC-AC (P = 0.01), AC-AM (P = 7.1E−05), YM-AM (P = 2.8E−07). Significance was determined using one-way ANOVA. *P < 0.05, ****P < 0.0001, ns P > 0.05. h Proportions of lung neutrophils were measured by flow cytometry, gated on CD45+ cells. Each group contains six mice. Data expressed as % of neutrophils (mean ± SD). YC-AC (P = 0.02), AC-AM (P = 1.1E−03), YM-AM (P = 1.6E−05). Significance was determined using one-way ANOVA. *P < 0.05, **P < 0.01, ****P < 0.0001, ns P > 0.05. i Quantity of neutrophils in per 0.1 g lung. Each group contains six mice. Data expressed as number of neutrophils (mean ± SD). YC-AC (P = 0.02), AC-AM (P = 3.5E−06), YM-AM (P = 1.7E−07). Significance was determined using one-way ANOVA. *P < 0.05, ****P < 0.0001, ns P > 0.05. Source data are provided as a Source Data file.