Fig. 6: Neutrophils of aged male mice suppress the stemness of CD8+ T cells. | Nature Communications

Fig. 6: Neutrophils of aged male mice suppress the stemness of CD8+ T cells.

From: Aging-induced immune microenvironment remodeling fosters melanoma in male mice via γδ17-Neutrophil-CD8 axis

Fig. 6

Representative images and quantitative results of lung metastases in aged (a) and young (b) mice with anti-Ly6G or isotype (control) treated. Each group contains six mice. Data expressed as mean ± SD. P = 8.3E−03 (a), P = 0.63 (b). Significance was determined using an unpaired two-tailed Student’s t test. **P < 0.01, ns P > 0.05. c Heatmap showing representative GO terms and pathways of CD8+ T cells across AC, YC, AM, and YM groups, calculated by GSVA packages. The color key from white to red indicates expression levels from low to high. d MFI of TCF1 in lung CD8+ T cells from AM/AM-anti-Ly6G groups, which were measured by flow cytometry, gated on CD45+CD8+ cells. Each group contains six mice. Data expressed as MFI of TCF1 (mean ± SD). P = 4.7E−03. Significance was determined using an unpaired two-tailed Student’s t test. **P < 0.01. Flow plots showing the proportions of Ki67+ (e), CD62L+CD44− (f), PD-1+Tim3+ (g), TNF+IFN-γ+ (h), GZMB+CD107a+ (i) in lung CD8+ T cells from AM anti-Ly6G/control groups measured by flow cytometry, gated on CD45+CD8+ cells. Each group contains six mice. Data expressed as % of Ki67+CD8+, CD62L+CD44-CD8+, PD-1+Tim3+CD8+, TNF+IFN-γ+CD8+, GZMB+CD107a+CD8+ cells (mean ± SD). P = 0.01 (e), P = 9.7E−03 (f), P = 0.01 (g), P = 0.02 (h), P = 0.03 (i), P = 8.6E−03 (k). Significance was determined using an unpaired two-tailed Student’s t test. *P < 0.05, **P < 0.01. j UMAP plot of CD8+ T cells subsets from AM and YM groups, and stacked column chart showing the ratio of Stem cell like (PD-1+TCF1+TIM3−) and Exhausted (PD-1+TCF1-TIM3+) CD8+ T cells in AM and YM groups, based on scRNA-seq. The total population is the sum of stem cell-like and exhausted CD8+ T cells. k Flow plot showing the proportions of Stem cell like CD8+ T cells in lungs from AM anti-Ly6G/control groups measured by flow cytometry, gated on CD45+CD8+PD-1+ cells. Each group contains six mice. Data expressed as % of stem cell like cells (mean ± SD). Significance was determined using an unpaired two-tailed Student’s t test. **P < 0.01. Source data are provided as a Source Data file.

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