Fig. 7: Analysis of the overexpression and repression of the pfp-ifp operon.
From: A plasmid-chromosome crosstalk in multidrug resistant enterobacteria
A Effect of the overexpression of LysRpOXA-48 on the growth of CF13 and KPN15. AUC of plasmid-free and pOXA-48ΔlysR-carrying CF13 and KPN15, with expression of LysRpOXA-48 in trans from the pTet-LysR (n = 5 replicates for each strain/condition pairing). Experiment performed with increasing concentrations of inducer (aTc). B Effect of CRISPRi repression on the expression of the pfp-ifp operon. RT-qPCR results for pfp as proxy of the whole operon, normalized to the endogenous control rpoB. Percentages indicate the level of repression of the operon (expression levels of pfp in cells with non-targeting pFR56apm vs. in cells carrying pFR56apm targeting the pfp-ifp operon). Error bars represent the standard error of the mean (n = 2 biological replicates, each as the median of 3 technical replicates). C Effect of CRISPRi repression on the growth of plasmid-free, pOXA-48- and pOXA-48ΔlysR-carrying KPN15. Individual points show independent replicates (n = 16). Asterisks represent statistical significance of two-way ANOVA and post hoc Tukey’s tests (Supplementary Data 11): *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. In boxplots (A) and (C), horizontal lines inside boxes indicate median values, upper and lower hinges correspond to the 25th and 75th percentiles, and whiskers extend to observations within the 1.5x the interquartile range. Source data are provided as a Source Data file.
