Fig. 6: Immunomodulating and pro-resolving effects associated with the MSC-bioreactor perfusion. | Nature Communications

Fig. 6: Immunomodulating and pro-resolving effects associated with the MSC-bioreactor perfusion.

From: A proof-of-concept study in small and large animal models for coupling liver normothermic machine perfusion with mesenchymal stromal cell bioreactors

Fig. 6

Perfusate profiling indicated that the bioreactor-based NMP induced a broad modulation of several factors involved in inflammation and leukocyte recruitment, inflammation resolution, and liver cell regeneration. Raw data were adjusted based on circuit volume and liver weight. Results are expressed as log2-transformed fold change between the NMP+bioreactor group and the NMP group at each time point. Five independent replicates were analyzed for each experimental condition. Two-way RM ANOVA, followed by Tukey’s post hoc test. The color-coded scale illustrates the diverse magnitudes of fold change. Abbreviations: b-MSCs, MSC-bioreactor; CCL2/MCP-1, Chemokine C-C motif ligand 2/Monocyte Chemoattractant Protein-1; CCL3/MIP-1α, Chemokine C-C motif ligand 3/Macrophage Inflammatory Protein-1α; CCL5/RANTES, Chemokine C-C motif ligand 5/regulated on activation normal T cell expressed and secreted; CTGF, Connective Tissue Growth Factor; CXCL-1/GRO α, C-X-C Motif Chemokine Ligand 1/Growth-regulated protein α; CXCL5/LIX, C-X-C Motif Chemokine Ligand 5/Lipopolysaccharide-induced CXC chemokine; CXCL10/IP-10, C-X-C Motif Chemokine Ligand 10/Interferon gamma-induced protein 10; FC, Fold change; HGF, Hepatocyte growth factor; IFN-γ, Interferon-γ; IL-4, Interleukin 4; IL-6, Interleukin 6; IL-10, Interleukin 10; IL-13, Interleukin 13; IL-18, Interleukin 18; NMP, normothermic machine perfusion; TIMP-1, Tissue Inhibitor of Metalloproteinase 1; TNF-α, Tumor Necrosis Factor-α; sICAM-1, soluble Intercellular Adhesion Molecule-1; VEGF, Vascular Endothelial Growth Factor.

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