Fig. 7: MSC-bioreactor-based liver NMP in a large animal model. | Nature Communications

Fig. 7: MSC-bioreactor-based liver NMP in a large animal model.

From: A proof-of-concept study in small and large animal models for coupling liver normothermic machine perfusion with mesenchymal stromal cell bioreactors

Fig. 7

In this pilot study, livers procured from donor swines were subjected to 4 h-NMP using the Liver Assist® (XVIVO Perfusion AB) device equipped with MSC-bioreactors (n = 3). The results were compared to those obtained from standard porcine liver NMP (n = 3). The collected data clearly indicate that the MSC-bioreactor can be safely integrated into a perfusion device currently available for the clinical setting. No difference in vascular a flows and b resistances was observed between livers subjected to the bioreactor-based perfusion compared to those exposed to the standard NMP. Points denote mean ± SEM, n = 3 biologically independent replicates. Two-way RM ANOVA; p-value between experimental groups: HA flow p = 0.151; HA resistance p = 0.285; PV flow p = 0.901; PV resistance p = 0.334. c Lactate clearance was similar across experimental groups. Points denote mean ± SEM, n = 3 biologically independent replicates. Two-way RM ANOVA; p = 0.563. d Perfusate LDH concentration tended to be lower in the NMP+bioreactor group. Points denote each experimental unit, n = 3 biologically independent replicates. Two-way ANOVA, p = 0.064. Abbreviations: b-MSCs, MSC-bioreactor; HA, hepatic artery; LDH, lactate dehydrogenase; NMP, normothermic machine perfusion; PV, portal vein.

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