Fig. 3: Translocation of ATG proteins to SGs depends on the assembly of SGs under HS.
From: Stress granules sequester autophagy proteins to facilitate plant recovery from heat stress
a The 5-day-old ATG13a-GFP, ATG6-GFP, ATG5-GFP, and UBP1c-GFP plant were subjected to 22 °C (control), 38 °C (HS) or 50 μM CHX + 38 °C HS treatment for 1 h. The root tips of indicated samples were observed using a confocal microscope. Scale bar = 10 μm. b The 5-day-old transgenic plants expressing ATG13a-GFP, ATG6-GFP, or ATG5-GFP in either WT or ubp1abc mutant were subjected to HS treatment at 38 °C for 1 h. The Meristem zone of indicated samples was observed using a confocal microscope. Scale bar = 10 μm. c Statistical analysis of the number of foci signals in (b). Data represent mean ± SD; n = 10. Ten different 50 × 50 μm2 areas in the meristem zone were used for colocalization analysis. Statistical analysis was performed using a two-tailed unpaired Student’s t test. d The UBP1c-GFP and UBP1c-GFP/atg5-1 plants were subjected to treatment at 22 °C (control) or 38 °C (HS) for 1 h, followed by heat-stressed plants recovered at 22 °C. The Meristem zone of indicated samples was observed using a confocal microscope. Scale bar = 10 μm. e Statistical analysis of the number of SG in WT and atg5-1 at HS and HS recovery phase in (d). Data represent mean ± SD; n = 8. Eight different 20 × 20 μm2 areas in the meristem zone were used for colocalization analysis. f The 5-day-old ATG-GFP × UBP1c-mCherry plants were subjected to HS treatment at 38 °C for 1 h, followed by recovery at 22 °C for 6 h. The Meristem zone of indicated samples was observed using a confocal microscope. Scale bar = 10 μm. Source data are provided as a Source Data file.
