Fig. 1: SCM-198 enables the survival of ALF mice.

a Schematics of the TAA- or APAP-induced ALF mouse model used in this study. SCM-198 (10 mg/Kg body weight) or NAC (200 mg/Kg body weight) was given intraperitoneally at 0.5 hpi (hour post-TAA or APAP intoxication/injection, prophylactic) or 24 hpi (therapeutic). Survival of the mice was examined at 144 hpi, and serum/tissue samples were collected at 48 hpi for all experiments, unless stated otherwise. b Percent survival chart of WT mice after saline, NAC, or SCM-198 treatment given at 0.5 or 24 hpi (n = 10 per group). The p-values were determined using the Log-rank test. c Serum ALT and AST levels were measured at 48 hpi in WT mice treated with saline, NAC, or SCM-198 0.5 hpi or 24 hpi (for all bar graphs from left to right, n = 4,4,5, 5,4,7; 4,4,5; 5,4,7; 5,4,5; 4,4,5; 5,4,5; 4,4,5). d Serum ALT and AST levels in TAA or APAP intoxicated WT mice at the time of SCM-198 administration (24 + 0), or 6 and 12 h after SCM-198 administration (24 + 6, 24 + 12). (n = 4). e GSH concentrations in WT mice liver tissue at 48 hpi (n = 5). f Quantification analysis of necrosis areas on liver sections at 48 hpi (n = 6 mice). g Quantification of F4/80+ cells on liver sections at 48 hpi (n = 3 mice). h Plasma levels of IL-1β and TNF-α detected by ELISA at 48 hpi (n = 6 mice). i Quantification of Ki67 + cells on liver sections at 48 hpi (n = 3 mice). j Percentages of TUNEL + cells on liver sections at 48 dpi (n = 3 mice). Data in (c–j) are presented as mean ± SEM. The p-values were determined using t test, two tails, except in (b), which was determined using the Log-rank test. n.s., not significant. Source data are provided as a Source Data file.