Fig. 8: Hepatoprotection by SCM-198 in LPS/D-GalN-induced ALF mice.

a Schematic diagram of the LPS/D-GalN-induced ALF model. b Percent survival chart of LPS/D-GalN-induced ALF mice after saline, NAC, or SCM-198 treatment given at 0.5 or 24 hpi (n = 10 per group). The p values were determined using the Log-rank test. c Serum ALT and AST levels were measured at 48 hpi in LPS/D-GalN-induced ALF mice treated with saline, NAC, or SCM-198 (n = 5 mice). d Serum ALT and AST levels in LPS/D-GalN-induced ALF, at 24 + 0, 24 + 6, 24 + 12 h after SCM-198 administration. (n = 3 mice). e Representative photographs of LPS/D-GalN-induced ALF livers, 48 hpi. Black arrowheads indicate lesion areas. Scale bars, 5 mm. f Representative micrographs of HE staining on the section of LPS/D-GalN-induced ALF liver. Scale bars, 100 µm. Quantification analysis of necrosis areas on liver sections was from 3 animals. g Representative immunofluorescence images of F4/80 + cells, TUNEL + cells, or Ki67 + cells on liver sections, 48 hpi. Quantification analysis was performed on 3 animals. h Plasma levels of IL-1β and TNF-α detected by ELISA, 48 hpi (n = 5 mice). i Representative images of BODIPY 493/403, BODIPY 581/591, and TMRM staining on liver sections, 48 hpi. j Serum GSH levels detected by ELISA, 48 hpi (n = 5 mice). k Liver tissue NO content, relative to the saline-treated liver, 48 hpi (n = 5 mice). l Immunoblotting of NOS3(pS1177), CaMKII(pT285), SOD1, PCNA, Bcl2, Bax in LPS/D-GalN-induced ALF liver tissues. Mice were treated with saline or SCM-198 at 24 hpi, and samples were collected at 48 hpi. Similar results were obtained in 2 independent repeats. Data in (c, d, f–h, j, k) are presented as mean ± SEM. The p-values were determined using SPSS v20 for t test, two-tail analysis. n.s., not significant. Source data are provided as a Source Data file.