Fig. 2: In-depth analysis of stability on individual EwS cell lines.
From: Genomic and phenotypic stability of fusion-driven pediatric sarcoma cell lines
a Newly purchased A-673, MHH-ES-1, SK-ES-1, SK-N-MC, and TC-71 EwS wild type cell lines (_1) were kept in culture for six months (m6; _2), and 12 months (m12; _3). ATCC, American Type Culture Collection, DSMZ (German Collection of Microorganism and Cell Cultures). b Bar plot shows the Euclidean distance of all SNPs after 6 (m6 vs. m0) and 12 months (m12 vs. m0) of continuous culture. c Number of differentially methylated CpG sites (including differentially hypo- and hyper-methylated) for A-673, MHH-ES-1, SK-ES-1, SK-N-MC, and TC-71 after six (m6) and 12 months (m12) of continuous culture, using the respective initial time point (m0) values as reference. d Volcano plot of DEG comparing each EwS cell line with their m12 derivate. Red dots denote significant DEG (BH adjusted P < 0.01; |FC | > 1). e Relative variation in cell viability for each EwS cell line measured as the mean area under the curve (AUC) m12 vs. m0 for the extended drug library (20 compounds). f Ranking plots depicting the linear distribution of five EwS cell lines based on their evolution across different datasets after continuous culturing for 12 months. g Schematic illustration summarizing the findings of this study. Created in BioRender. Aranaz, F. (2023) BioRender.com/k87h648.
