Fig. 7: AKH secretion from APCs is regulated by amino acids.

A 1st instar fly larvae were transferred onto 2% agarose for 6 h and then onto different test foods containing 5% sucrose, 10% Tryptone (protein diet), or no nutrient (starvation diet). B–D Representative Ca²⁺ activities in the APCs of free-behaving larvae transferred between different diets. Time courses of average Ca²⁺ signal from multiple larvae were plotted as mean ± S.E.M. (shaded region). The gap between the two diets is the larvae transfer time which is ~1 min. E, F Representative Ca²⁺ images of the APCs of free-behaving larvae fed on different diets. G Quantification of Ca²⁺ activities in the APCs of free-behaving larvae. N = 10, 10, 10 larvae. Each dot represents an independent biological replicate. Ca²⁺ intensity (arbitrary units) was presented using a linear colour scale (minimum = 0, maximum = 255) (E, F). Ordinary one-way ANOVA with Dunnett’s multiple comparisons was used in (G). Data in (B–D) were plotted as mean ± S.E.M. Data in were plotted as mean ± S.E.M (B–D), or mean ± SD (G). Scale bars, 25 μm (E, F). Source data are provided as a Source Data file.