Fig. 3: Loss of OsINO80 leads to a decrease in the H3K27me3 level.

a Average density plots of the normalized H3, H3K4me3, H3K36me3, H3K4me2, H3K27me3, and H2Aub profiles of randomly selected putative T1 OsINO80 target genes (n = 3000) and the random control H2A.Z-enriched genes (without OsINO80 enrichment) selected on the basis of number and length in the wild-type (WT) and the osino80-2 mutant. The plots present the region from 1 kb upstream of TSS to 1 kb downstream of TTS. b Heatmaps of OsINO80, H2A.Z, and H3 distributions within randomly selected putative T1 OsINO80 target genes (n = 3000) in WT rice plants and the changes in H2A.Z, H3, H3K4me3, H3K36me3, H3K4me2, H3K27me3, and H2Aub distributions in the osino80-2 mutant (compared to the WT control), with rows ordered according to decrease in OsINO80 signal. c Heatmaps of random H2A.Z-enriched genes (without OsINO80 enrichment) selected on the basis of number and length as the control for (b), with rows ordered according to decrease in H2A.Z signal. b, c Heatmaps of the ChIP-seq signals were generated for the region from 1 kb upstream of TSS to 1 kb downstream of TTS. Statistic significances were determined by two-sided Welch Two Sample t-test.