Fig. 6: KBrO3-induced 8-oxodG mutation and lesion strand bias on leading/lagging and transcribed/non-transcribed strands in human cells.
From: Contributing factors to the oxidation-induced mutational landscape in human cells
A Mean values of G > T, G > C, and G > A mutations on the leading strand (red bars) or on the lagging strand (blue bars). B Mean values of G > T, G > C, and G > A mutations on the non-transcribed strand (red bars) transcribed strand (blue bars). circles represent values from each biologically independent sequenced genome (n = 4, 6, and 1 for WT, HMCES-/-, and POLH-/-, respectively) or technical replicate measurements of KBrO3-induced lesions (n = 2). The bar graph below each plot represents the log2(red/blue) value of the for the event per Mb bars. Significant differences between strands are indicated with p-values determined by two-sided paired t-test for WT and HMCES-/- genomes. p-values (calculated using GraphPad Prism) for G base mutation types in the POLH-/- sample were calculated by two-sided Chi-square against the null hypothesis of equal representation of mutations on both DNA strands.
