Fig. 1: Development of cgSHAPE-seq and anti-viral RIBOTAC. | Nature Communications

Fig. 1: Development of cgSHAPE-seq and anti-viral RIBOTAC.

From: Chemical-guided SHAPE sequencing (cgSHAPE-seq) informs the binding site of RNA-degrading chimeras targeting SARS-CoV-2 5’ untranslated region

Fig. 1

a RNA secondary structures in SARS-CoV-2 5’ UTR and the pipeline in identification of the ligand binding site and the development of RNA-degrading chimeras. b Principle of cgSHAPE-seq for identifying small molecule binding sites in four steps. Step 1: Synthesis of FAI conjugated chemical probe. Step 2: Chemical-guided acylation at the 2’-OH of ribose at the binding site. Step 3: Reverse transcription in the presence of Mn2+ creates single-point mutations at the acylation site. Step 4: Mutational profiling and quantification identify the putative binding sites. c RNA-degrading chimeras (RIBOTACs) recruit RNase L at the target RNA to degrade viral RNAs. Created in BioRender. Tang, Z. (2024) https://BioRender.com/w93l410. Elements of this figure were provided by figdraw.com.

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