Fig. 3: Bidirectional DA dynamics upon sensory-driven sniffing.
From: Dopaminergic signaling to ventral striatum neurons initiates sniffing behavior
a Setup for measurement of respiration during GRABDA photometry. Some aspects created in BioRender. Wesson, D. (2024) https://BioRender.com/w71o057. b Example 465 nm GRABDA and 405 nm UV signals recorded in the TuS upon odor delivery. resp = respiration. c–e Odor-evoked sniffing and GRABDA in the TuS (c), NAcSh (d), and NAcC (e). Sniffing frequency of TuS (ci), NAcSh (di), and NAcC (ei) implanted mice across repeated odors. Mean shown in black, individual mice in light gray. Averaged GRABDA in TuS (cii), NAcSh (dii), and NAcC (eii). Averaged peak of z-scored GRABDA in the TuS (ciii), NAcSh (diii), and NAcC (eiii). Mean shown in black with individual mice across trials colored as in Fig. 2d. Correlations between GRABDA and average sniff frequency during bout in TuS, (r = 0.65, p < 0.0001, civ) and NAcSh (r = 0.51, p = 0.002, div) but not in NAcC (r = −0.14, p = 0.414, eiv; Pearson correlations). f–h Buzz-evoked sniffing and GRABDA from same mice in TuS (f), NAcSh (g), and NAcC (h). Data in (f–h) organized as in c–e. Correlation between GRABDA and sniffing in the TuS, (r = 0.43, p = 0.011, fiv), but not NAcSh (during buzz: r = −0.17, p = 0.316; upon buzz offset: r = −0.074, p = 0.673, giv) or NAcC (r = −0.22, p = 0.194, hiv; Pearson correlations). i Comparison of evoked GRABDA in TuS, NAcSh, and NAcC. Data are mean ± SEM. Individual points = individual mice. ****p < 0.0001, ***p < 0.001, **p < 0.01. Data shown in (cii), (dii), (eii), (fii), (gii), and (hii) are smoothed for visualization. Source data are provided as a Source Data file.
