Fig. 3: Synthetic ecteinascidins induce cell cycle arrest and apoptosis. | Nature Communications

Fig. 3: Synthetic ecteinascidins induce cell cycle arrest and apoptosis.

From: Pan-inhibition of super-enhancer-driven oncogenic transcription by next-generation synthetic ecteinascidins yields potent anti-cancer activity

Fig. 3

a Metastatic melanoma cells were treated with either vehicle (DMSO), lurbinectedin, ecubectedin or PM54 (1xIC50 concentration, 48 h) and allowed to grow for additional 10 days in the absence of drugs. Results are shown as the mean colony numbers ± SD for three biological triplicates. Ordinary one-way ANOVA using Dunnett’s multiple comparisons test was used to determine the p-values (vs. DMSO). Source data are provided as a Source Data file. b Metastatic melanoma cells were incubated with CellTrace and subsequently treated with either vehicle (DMSO), lurbinectedin, ecubectedin or PM54 (1xIC50 concentration, 72 h). Quantifications of populations with high CellTrace signal in DMSO or drug-treated cells are shown as mean values ± SD for three biological triplicates. Proliferative cells show low CellTrace signal while non proliferative cells show high CellTrace signal. Ordinary one-way ANOVA using Dunnett’s multiple comparisons test was used to determine the p-values (vs. DMSO). Source data are provided as a Source Data file. c 501mel cells were treated with either vehicle (DMSO), lurbinectedin, ecubectedin or PM54 (1xIC50 concentration, 72 h). Cell cycle was studied by propidium iodide staining and flow cytometry, and results are shown as mean values ± SD for three biological triplicates. Source data are provided as a Source Data file. d Metastatic melanoma cells were treated with either vehicle (DMSO), lurbinectedin, ecubectedin or PM54 (1xIC50 concentration, 72 h). Apoptosis was studied by flow cytometry using annexin V-APC staining. Results are shown as mean values ± SD for three biological triplicates. Ordinary one-way ANOVA using Dunnett’s multiple comparisons test was used to determine the p-values (vs. DMSO). Source data are provided as a Source Data file. e MM029 and MM099 metastatic melanoma cells were treated with either vehicle (DMSO), lurbinectedin, ecubectedin or PM54 (1xIC50 concentration, 48 h). Invasion was determined using Boyden chamber assays. Results are shown as mean values of coverage index +/- SD for three biological triplicates. Ordinary one-way ANOVA using Dunnett’s multiple comparisons test was used to determine the p-values (vs. DMSO). Source data are provided as a Source Data file.

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