Fig. 3: G4 DNA at the human PKD1 locus in normal and ADPKD tissue. | Nature Communications

Fig. 3: G4 DNA at the human PKD1 locus in normal and ADPKD tissue.

From: G-quadruplex stabilization provokes DNA breaks in human PKD1, revealing a second hit mechanism for ADPKD

Fig. 3

A, B Normal human kidney tissue sections labeled with SG4 nanobody (green) and dCAS9 with sgRNAs to PKD1 (red). B Boxed region in (A) enlarged showing G4 DNA and PKD1 colocalization (arrows). C, D Human ADPKD tissue sections were labeled with SG4 nanobody against G4 DNAs (green) and dCAS9 with sgRNAs to PKD1 (red). D Boxed region in (C) is enlarged to show G4 DNA and PKD1 co-localization (arrows). E–H are controls. E, F normal human kidney tissue sections labeled with mutated nanobody (SG4mut-R105A) (green) and dCAS9 with sgRNAs to PKD1 (red). G, H human ADPKD tissue sections labeled with SG4 nanobody (green) and dCAS9 without sgRNAs (red). Boxed region in (E, G) are expanded in (F, H), respectively. Scale bars in (A, C, E, G = 50 microns). Scale bars in (B, D, F, H = 10 microns).

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