Fig. 7: Delipidation of ATG8 is critical for autophagy in C. reinhardtii.
From: ATG8 delipidation is not universally critical for autophagy in plants
A Western blot detection of endogenous ATG8 and mCherry–CrATG8 transgene expression in the WT, cratg4, and cratg5 backgrounds. Ponceau S staining was used as a loading control. The cells were grown in the liquid TAP medium under standard conditions and harvested at a density of 1–2 × 107 cells ml−1. B Senescence phenotype of the transgenic Chlamydomonas lines grown on the TAP medium for 40 days. The light color of colonies indicates nutrient deficiency sensitivity caused by dysfunctional autophagy. Scale bar, 1 cm.
