Fig. 3: Specificity of human DNA licensing. | Nature Communications

Fig. 3: Specificity of human DNA licensing.

From: Reconstitution of human DNA licensing and the structural and functional analysis of key intermediates

Fig. 3

Specificity of the pre-RC assay was assessed under (a) low and (b) high salt conditions by sequentially omitting loading factors hORC1-5 (lane 1), hCDC6 (lane 2) or hCDT1 (lane 3) compared to a reaction containing all pre-RC proteins (lane 4). SDS-PAGE gels are representative of three independent biological replicates. c Pre-RC assay under low and high salt wash conditions was carried out with hB2-Lamin and yARS1 DNA. Highlighting that human DNA licensing can occur on non-human DNA. SDS-PAGE gel is representative of two independent biological replicates. Source data are provided as a Source Data file.

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