Fig. 7: The hORC1-hCDC6 interface becomes restructured in the hOCCM and hCDC6 ATPase is crucial for complex disassembly. | Nature Communications

Fig. 7: The hORC1-hCDC6 interface becomes restructured in the hOCCM and hCDC6 ATPase is crucial for complex disassembly.

From: Reconstitution of human DNA licensing and the structural and functional analysis of key intermediates

Fig. 7

a Observed stabilisation of an hORC1 helix formed between residues 660–674, which is unstructured before hCDC6 (grey) binding as evidenced by docking hORC1 (PDB: 7JPS) in light grey and hORC1 of hOCCM in green. b Purified hORC1-5WT and hORC1-5 ORC1 Walker B mutant (hORC1-5E621Q), hCDC6WT and hCDC6 Walker B mutant (hCDC6E285Q). c The pre-RC assay was carried out under low and high salt wash conditions with hORC1-5WT, hORC1-5E621Q, hCDC6WT or hCDC6E285Q. SDS-PAGE gel is representative of three independent biological replicates. Source data are provided as a Source Data file.

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