Fig. 9: Five hMCM2-7 WHDs make contact with hORC1-5-hCDC6. | Nature Communications

Fig. 9: Five hMCM2-7 WHDs make contact with hORC1-5-hCDC6.

From: Reconstitution of human DNA licensing and the structural and functional analysis of key intermediates

Fig. 9

a Five hMCM (grey) WHDs (coloured) form contacts with hORC1-5-hCDC6 in hOCCM, contrasting with only four inter-subunit interactions in yOCCM (PDB: 5V8F). The conserved interfaces are highlighted in brackets, and the unique interfaces are circled. b Pre-RC assay under low and high salt wash conditions was carried out with WT and hMCM2ΔC (hMCM2-WHD truncated mutant) hMCM2-7. SDS-PAGE gel is representative of two independent biological replicates. c Comparison between yeast and human MCM2 ___domain organisation. hMCM2ΔC lacks aa826-904, the WHD ___domain (red). d Purification of the hMCM5ΔC truncation mutant results in sub-stoichiometric hMCM2-7 complexes compared to purified hMCM2-7WT. e Structural comparison of the human and yeast MCM6-CDT1 interface and sequence alignment of the corresponding region. Source data are provided as a Source Data file.

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