Fig. 1: Sex biases identified in inadequately host-filtered human tumor tissue data.

a RPCA of microbial relative abundance quantification from tumor samples in the Hartwig Medical Foundation Database, which was originally subject to GRCh38.p7 filtration exclusively. Statistically significant differences were found between male and female groups (PERMANOVA; pseudo-F = 65.4, p = 0.00025). b Identical dataset and pre-processing steps done in a but with the addition of the T2T-CHM13v2.0 reference genome in host filtration. Differences were not statistically significant between male and female groups (PERMANOVA; pseudo-F = 1.23, p = 0.29).