Fig. 6: CD26⁺ TSPCs contribute to ectopic bone formation.

a GO pathway analysis of upregulated genes toward chondrocyte lineage based on their common kinetics through pseudotime. Hypergeometric test, FDR correction. b Pseudotime analysis of expression of chondrocyte markers in each state. c Scheme for punched model establishment and tamoxifen (TMX) administration. d Immunofluorescent staining of Sox9 and tdT. n = 5. Scale bar: 100 μm. e Quantification analysis of Sox9⁺ cell and tdT⁺Sox9⁺ cell rate. n = 5 biological replicates. One way ANOVA with Turkey’s test, Top: F = 257.7; Buttom: F = 190.0. f Immunofluorescent staining of Sp7 and tdT. n = 5. Scale bar: 100 μm. g Quantification analysis of Sp7⁺ cell and tdT⁺Sp7⁺ cell rate. n = 5 biological replicates. One way ANOVA with Turkey’s test, Top: F = 103.5; Buttom: F = 152.5. Data shown are mean ± SEM. Source data are provided as a Source Data file.