Fig. 2: Specific targeting of hypoxic NPC by Cy5.5-HFn in vitro and in C17 PDX models. | Nature Communications

Fig. 2: Specific targeting of hypoxic NPC by Cy5.5-HFn in vitro and in C17 PDX models.

From: Hypoxia-tropic delivery of nanozymes targeting transferrin receptor 1 for nasopharyngeal carcinoma radiotherapy sensitization

Fig. 2

a IF analysis of NPC tissue from C17 PDX models showing the co-localization of Cy5.5-HFn with HIF-1α. Scale bar, 50 μm. b Distribution of Cy5.5-HFn staining intensity in hypoxic and normoxic regions of NPC tissue based on a (n = 8 randomly selected fields from different tissues). c Schematic representation of the intravenous (i.v.) injection of Cy5.5-HFn into C17 PDX mouse model. d In vivo fluorescence imaging of C17 PDX model mice at different time points before and after the injection of Cy5.5-HFn. e Quantitative analysis of Cy5.5-HFn signal in the livers and tumors based on d (n = 3 mice). f Distribution of Cy5.5-HFn signal intensity in major organs and tumors at 48 h post-injection (n = 3 mice). Inset images show ex vivo fluorescence images. g Co-localization IF analysis of Cy5.5-HFn with Pimo-tracked hypoxic regions in C17 PDX tumor tissue at 12 h post-injection. Scale bar, 50 μm. h Distribution of average fluorescence signals of Cy5.5-HFn in vascular regions and hypoxic and normoxic areas of non-vascular regions, based on g (n = 6 randomly selected fields from the same tissue). Data are shown as mean ± SEM. Paired, two-tailed Student’s t test in b and repeated-measures one-way ANOVA with Geisser-Greenhouse correction and with Tukey’s multiple comparisons test in h were used to calculate P values. Source data are provided as a Source Data file.

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