Fig. 2: Ovoid neurons exhibit specialized long-range axonal targets.
From: Atypical hippocampal excitatory neurons express and govern object memory
A Schematic of subtype-specific labeling strategy in Ly6g6e-IRES-Cre mouse line. B Representative image of viral expression in the subiculum for strategy in (A). Note that images are oriented so that primary apical dendrites of pyramidal cells extend upwards. Independently repeated across 2 sections from 4 animals. Scale bar: 100 µm. C Atlas illustrating the anterior thalamic nuclei (ATN). D Representative projections to the anterior thalamic nuclei for CAG-FLEX-EGFP injection scheme shown in (A). Scale bar: 300 µm. E Axonal reconstruction of a single subiculum neuron projecting to the ATN. F As in (E), but for a single neuron with projections elsewhere. G Axonal reconstructions for downstream brain regions. H Axonal length in downstream brain regions (72 non-ATN-projecting cells, 12 ATN-projecting cells, p = 1.9E-12, two-sided Mann-Whitney U test). Data are presented as mean values ± SEM. ACB Nucleus Accumbens, ATN Anterior Thalamic Nuclei, HIP Hippocampal Region, LS Lateral Septal Nucleus, MBO Mammillary Body, MSC Medial Septal Complex, RHP Retrohippocampal Region, RSP Retrosplenial Area.
