Fig. 1: Identification of the intermediate complex investigated in this study.
From: Structure and function of a near fully-activated intermediate GPCR-Gαβγ complex
a Proposed activation model of GPCR, including inactive states S1 and S2, pre-coupled complex (S3-Gαsβγ), intermediate complex (S4-Gαsβγ), and the fully activated complex (S5-Gαsβγ), in which S4-Gαsβγ was highlighted in teal for this study. b In reference to Supplementary Fig. 1, the deconvoluted conformational profiles probed by19F-qNMR for the R291A construct were presented as a function of Gαsβγ and mini-Gαsβγ. 19F-qNMR spectra of WT* and R291A were used as the benchmarks for S1 through S5, adapted from the reference (Wang et al, 2023, Nature Commun). The spectrum of WT*+mini-Gαsβγ + NECA + NB35 represents the feature for PDB: 6GDG. c The population distributions of conformational states for R291A+mini-Gαsβγ+NB35, R291A + Gαsβγ, and WT*+mini-Gαsβγ+NB35 (S5-Gαsβγ). The source data for these population distributions are included in the Source Data file. Data with error bars are presented as state population±SD. The SD values were determined based on spectral S/Ns and fitting errors of the deconvolutions. d Cryo-EM density map of the intermediate S4-mini-Gαsβγ complex. e Ribbon representation of S4-mini-Gαsβγ. f Highlighted interfacial section of S4-mini-Gαsβγ.
